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homo sapiens female a375  (ATCC)


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    ATCC homo sapiens female a375
    Homo Sapiens Female A375, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5224 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/homo+sapiens+female+a375/A-375/10__7554_slash_elife__73430-221-62-66
    Average 99 stars, based on 5224 article reviews
    homo sapiens female a375 - by Bioz Stars, 2026-09
    99/100 stars

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    Article Title: Experiments from unfinished Registered Reports in the Reproducibility Project: Cancer Biology
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Cell line (Homo sapiens, male) PC9 Sigma- Aldrich cat# 90071810; RRID:CVCL_B260 lot# 14A030 Chemical compound, drug erlotinib Cayman Chemical cat# 10,483 lot# 0459700- 31 Chemical compound, drug erlotinib- HCl LC Laboratories cat# E- 4007 lot# BBE- 108 Software, algorithm Cellometer Auto T4 Software Nexcelom Bioscience RRID:SCR_021656 Version 3.1.1 Software, algorithm Image Studio Software LI- COR Biosciences RRID:SCR_015795 Antibody rabbit- anti- phosphoEGFR (Y1068) Cell Signaling Technology cat# 3777; clone D7A5; RRID:AB_2096270 1:1000 dilution Antibody mouse anti- GAPDH Thermo Fisher Scientific cat# MA5- 15738; clone GA1R; RRID:AB_10977387 1:2000 dilution Antibody IRDye 680RD- conjugated donkey anti- mouse LI- COR Biosciences cat# 926- 68072; RRID:AB_10953628 1:15,000 dilution Antibody IRDye 800CWconjugated donkey antirabbit LI- COR Biosciences cat# 926- 32213; RRID:AB_621848 1:15,000 dilution Software, algorithm 2,100 Bioanalyzer Software Agilent Technologies RRID:SCR_019389 Version 1.03 Cell line (Homo sapiens, male) U87MG ATCC cat#HTB- 14; RRID:CVCL_0022 Cell line (Homo sapiens, male) HMVEC Lonza CC- 2516 Cell line (Homo sapiens, male) GSC83 doi:10.1038/nature09557 RRID:CVCL_A9TL Shared by Ricci- Vitiani lab, Istituto Superiore di Sanità Cell line (Homo sapiens, female) HMEC Applied Biological Materials cat# T0454; RRID:CVCL_B0CI lot# RZ824921; infected with SV40 large T and small T antigen Recombinant DNA reagent pRetroX- IRES- ZsGreen1empty vector Clontech cat# 632,520 Recombinant DNA reagent pRetroX- FLAGGNAO1WT- IRESZsGreen1 This paper Recombinant DNA reagent pRetroX- FLAGGNAO1R243H- IRESZsGreen1 This paper Cell line (Homo sapiens, female) Phoenix ATCC cat# CRL- 3213; RRID:CVCL_ H716 Antibody mouse anti- FLAG Sigma- Aldrich cat# F1804; clone M2; RRID:AB_262044 1:500 dilution Antibody mouse anti-β-ACTIN Sigma- Aldrich cat# A5441; clone AC- 15; RRID:AB_476744 1:1000 dilution Errington et al. eLife 2021;10:e73430. .. DOI: https://doi.org/10.7554/eLife.73430 15 of 27 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody HRP- conjugated rabbit anti- mouse Abcam cat# ab6728; RRID:AB_955440 1:10,000 dilution Software, algorithm FACSDiva BD Biosciences RRID:SCR_016722 Version 6.1.2 Software, algorithm ImageJ doi:10.1038/nmeth.2089 RRID:SCR_003070 Version 1.51p Cell line (Homo sapiens, female) D04 ABN Cell Line Bank, QIMR Berghofer Medical Research Institute RRID:CVCL_H604 Cell line (Homo sapiens, female) A375 ATCC cat# CRL- 1619; RRID:CVCL_0132 Chemical compound, drug sorafenib Selleckchem cat# S7397 Chemical compound, drug SB590885 Selleckchem cat# S2220 Chemical compound, drug PD184352 Selleckchem cat# S1020 Antibody mouse anti- phosphoERK1/2 (T202/Y204) Cell Signaling Technology cat# 9106; clone E10; RRID:AB_331768 1:1000 dilution Antibody Rabbit anti- ERK1/2 Cell Signaling Technology cat# 9102; RRID:AB_330744 1:1000 dilution Cell line (Homo sapiens, female) RPMI- 7951 ATCC cat# HTB- 66; RRID:CVCL_1666 Cell line (Homo sapiens, male) OUMS- 23 JCRB cat# JCRB1022; RRID:CVCL_3088 Cell line (Homo sapiens, female) HT- 29 ATCC cat# HTB- 38; RRID:CVCL_0320 Chemical compound, drug PLX4720 Selleckchem cat# S1152 Chemical compound, drug MAP3K8 kinase inhibitor EMD Millipore cat# 616,373 Antibody rabbit anti- phospho MEK1/2 (S217/221) Cell Signaling Technology cat# 9154; clone 41G9; RRID:AB_2138017 1:1000 dilution Antibody mouse anti- ERK1/2 Cell Signaling Technology cat# 4696; clone L34F12; RRID:AB_390780 1:1000 dilution Antibody rabbit anti- MEK1/2 Cell Signaling Technology cat# 8727; clone D1A5; RRID:AB_10829473 1:1000 dilution Antibody rabbit anti- Vinculin Sigma- Aldrich cat# V4139; RRID:AB_262053 1:20,000 dilution Antibody HRP- conjugated goat anti- rabbit Cell Signaling Technology cat# 7074; RRID:AB_2099233 1:1000 dilution Antibody HRP- conjugated horse anti- mouse Cell Signaling Technology cat# 7076; RRID:AB_330924 1:1000 dilution Strain, strain background (M. musculus, C.BKalghb/lcrCrl, female) C.B- 17 wild- type Charles River RRID:IMSR_CRL:251 Continued on next page Continued Errington et al. eLife 2021;10:e73430. ..



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    99
    ATCC homo sapiens female a375
    Homo Sapiens Female A375, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/homo+sapiens+female+a375/A-375/10__7554_slash_elife__73430-221-62-66
    Average 99 stars, based on 1 article reviews
    homo sapiens female a375 - by Bioz Stars, 2026-09
    99/100 stars
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